Part:BBa_I739014

Single regulated intermediate for P22 cII +LVA (I739101.B0034.C0053)

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) under control of the double promoter BBa_I739101 followed by the ribosome binding site BBa_B0034. Please notice that there is no terminator in this construct. If you plan to use this construct for double regulated p22cII production, you should combine it with an appropriate terminator (e.g. BBa_B0015).

Mode of Action

P22 cII production can be repressed by TetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, TetR action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and is an intermediate biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. Together with Part 5, this Biobrick forms the composite part BBa_I739021.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 275